T Cell Activation is Determined by the Number of Presented Antigens

[Image: see text] Antigen recognition is a key event during T cell activation. Here, we introduce nanopatterned antigen arrays that mimic the antigen presenting cell surface during T cell activation. The assessment of activation related events revealed the requirement of a minimal density of 90–140...

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Autores principales: Deeg, Janosch, Axmann, Markus, Matic, Jovana, Liapis, Anastasia, Depoil, David, Afrose, Jehan, Curado, Silvia, Dustin, Michael L., Spatz, Joachim P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2013
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828117/
https://www.ncbi.nlm.nih.gov/pubmed/24117051
http://dx.doi.org/10.1021/nl403266t
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author Deeg, Janosch
Axmann, Markus
Matic, Jovana
Liapis, Anastasia
Depoil, David
Afrose, Jehan
Curado, Silvia
Dustin, Michael L.
Spatz, Joachim P.
author_facet Deeg, Janosch
Axmann, Markus
Matic, Jovana
Liapis, Anastasia
Depoil, David
Afrose, Jehan
Curado, Silvia
Dustin, Michael L.
Spatz, Joachim P.
author_sort Deeg, Janosch
collection PubMed
description [Image: see text] Antigen recognition is a key event during T cell activation. Here, we introduce nanopatterned antigen arrays that mimic the antigen presenting cell surface during T cell activation. The assessment of activation related events revealed the requirement of a minimal density of 90–140 stimulating major histocompatibility complex class II proteins (pMHC) molecules per μm(2). We demonstrate that these substrates induce T cell responses in a pMHC dose-dependent manner and that the number of presented pMHCs dominates over local pMHC density.
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spelling pubmed-38281172013-11-14 T Cell Activation is Determined by the Number of Presented Antigens Deeg, Janosch Axmann, Markus Matic, Jovana Liapis, Anastasia Depoil, David Afrose, Jehan Curado, Silvia Dustin, Michael L. Spatz, Joachim P. Nano Lett [Image: see text] Antigen recognition is a key event during T cell activation. Here, we introduce nanopatterned antigen arrays that mimic the antigen presenting cell surface during T cell activation. The assessment of activation related events revealed the requirement of a minimal density of 90–140 stimulating major histocompatibility complex class II proteins (pMHC) molecules per μm(2). We demonstrate that these substrates induce T cell responses in a pMHC dose-dependent manner and that the number of presented pMHCs dominates over local pMHC density. American Chemical Society 2013-10-11 2013-11-13 /pmc/articles/PMC3828117/ /pubmed/24117051 http://dx.doi.org/10.1021/nl403266t Text en Copyright © 2013 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Deeg, Janosch
Axmann, Markus
Matic, Jovana
Liapis, Anastasia
Depoil, David
Afrose, Jehan
Curado, Silvia
Dustin, Michael L.
Spatz, Joachim P.
T Cell Activation is Determined by the Number of Presented Antigens
title T Cell Activation is Determined by the Number of Presented Antigens
title_full T Cell Activation is Determined by the Number of Presented Antigens
title_fullStr T Cell Activation is Determined by the Number of Presented Antigens
title_full_unstemmed T Cell Activation is Determined by the Number of Presented Antigens
title_short T Cell Activation is Determined by the Number of Presented Antigens
title_sort t cell activation is determined by the number of presented antigens
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828117/
https://www.ncbi.nlm.nih.gov/pubmed/24117051
http://dx.doi.org/10.1021/nl403266t
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