Patch cloning method for multiple site-directed and saturation mutagenesis

BACKGROUND: Various DNA manipulation methods have been developed to prepare mutant genes for protein engineering. However, development of more efficient and convenient method is still demanded. Homologous DNA assembly methods, which do not depend on restriction enzymes, have been used as convenient...

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Detalles Bibliográficos
Autores principales: Taniguchi, Naohiro, Nakayama, Sayumi, Kawakami, Takashi, Murakami, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829206/
https://www.ncbi.nlm.nih.gov/pubmed/24164925
http://dx.doi.org/10.1186/1472-6750-13-91

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829206/
https://www.ncbi.nlm.nih.gov/pubmed/24164925
http://dx.doi.org/10.1186/1472-6750-13-91

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