Cargando…

Patch cloning method for multiple site-directed and saturation mutagenesis

BACKGROUND: Various DNA manipulation methods have been developed to prepare mutant genes for protein engineering. However, development of more efficient and convenient method is still demanded. Homologous DNA assembly methods, which do not depend on restriction enzymes, have been used as convenient...

Descripción completa

Detalles Bibliográficos
Autores principales:	Taniguchi, Naohiro, Nakayama, Sayumi, Kawakami, Takashi, Murakami, Hiroshi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829206/ https://www.ncbi.nlm.nih.gov/pubmed/24164925 http://dx.doi.org/10.1186/1472-6750-13-91

Ejemplares similares

A simple, flexible and efficient PCR-fusion/Gateway cloning procedure for gene fusion, site-directed mutagenesis, short sequence insertion and domain deletions and swaps
por: Atanassov, Ivan I, et al.
Publicado: (2009)

An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol
por: Liu, Huanting, et al.
Publicado: (2008)

Golden Mutagenesis: An efficient multi-site-saturation mutagenesis approach by Golden Gate cloning with automated primer design
por: Püllmann, Pascal, et al.
Publicado: (2019)

Scarless and site-directed mutagenesis in Salmonella enteritidis chromosome
por: Cox, Mandy M, et al.
Publicado: (2007)

A mutant screening method by critical annealing temperature-PCR for site-directed mutagenesis
por: Liu, Ying, et al.
Publicado: (2013)

A simple and efficient seamless DNA cloning method using SLiCE from Escherichia coli laboratory strains and its application to SLiP site-directed mutagenesis
por: Motohashi, Ken
Publicado: (2015)

MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR
por: Fushan, Alexey A, et al.
Publicado: (2009)

Complementary annealing mediated by exonuclease: a method for seamless cloning and conditioning site-directed mutagenesis
por: Sun, Shuhui, et al.
Publicado: (2015)

Direct fluorescent labelling of clones by DOP PCR
por: Backx, Liesbeth, et al.
Publicado: (2008)

GAL1-SceI directed site-specific genomic (gsSSG) mutagenesis: a method for precisely targeting point mutations in S. cerevisiae
por: Piccirillo, Sarah, et al.
Publicado: (2011)

New approach for fish breeding by chemical mutagenesis: establishment of TILLING method in fugu (Takifugu rubripes) with ENU mutagenesis
por: Kuroyanagi, Miwa, et al.
Publicado: (2013)

T5 exonuclease-dependent assembly offers a low-cost method for efficient cloning and site-directed mutagenesis
por: Xia, Yongzhen, et al.
Publicado: (2019)

Site Saturation Mutagenesis Applications on Candida methylica Formate Dehydrogenase
por: Özgün, Gülşah P., et al.
Publicado: (2016)

REPLACR-mutagenesis, a one-step method for site-directed mutagenesis by recombineering
por: Trehan, Ashutosh, et al.
Publicado: (2016)

A visual method for direct selection of high-producing Pichia pastoris clones
por: Hu, Fan, et al.
Publicado: (2011)

A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system
por: Bhat, Sushant, et al.
Publicado: (2020)

Promoter analysis by saturation mutagenesis
por: Baliga, Nitin S
Publicado: (2001)

Improving the thermostability of alpha-amylase by combinatorial coevolving-site saturation mutagenesis
por: Wang, Chenghua, et al.
Publicado: (2012)

Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies
por: Edelheit, Oded, et al.
Publicado: (2009)

Site-specific genomic (SSG) and random domain-localized (RDL) mutagenesis in yeast
por: Gray, Misa, et al.
Publicado: (2004)

Engineering of 3-ketosteroid-∆(1)-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates
por: Mao, Shuhong, et al.
Publicado: (2018)

LUMI-PCR: an Illumina platform ligation-mediated PCR protocol for integration site cloning, provides molecular quantitation of integration sites
por: Dawes, Joanna C., et al.
Publicado: (2020)

Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates
por: Sanchis, Joaquin, et al.
Publicado: (2008)

Economical analysis of saturation mutagenesis experiments
por: Acevedo-Rocha, Carlos G., et al.
Publicado: (2015)

EMS mutagenesis in mature seed-derived rice calli as a new method for rapidly obtaining TILLING mutant populations
por: Serrat, Xavier, et al.
Publicado: (2014)

A novel Gateway(®)-compatible binary vector allows direct selection of recombinant clones in Agrobacterium tumefaciens
por: Traore, Sy Mamadou, et al.
Publicado: (2011)

Pyrite cloning: a single tube and programmed reaction cloning with restriction enzymes
por: Fischer, Matthew D., et al.
Publicado: (2018)

A high-throughput method to detect Plasmodium falciparum clones in limiting dilution microplates
por: Lyko, Brian, et al.
Publicado: (2012)

An improved method for undertaking limiting dilution assays for in vitro cloning of Plasmodium falciparum parasites
por: Butterworth, Alice S, et al.
Publicado: (2011)

Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis
por: Hussain, Hasnain, et al.
Publicado: (2016)

Efficient method for site-directed mutagenesis in large plasmids without subcloning
por: Hallak, Louay K., et al.
Publicado: (2017)

Sequencing methods and datasets to improve functional interpretation of sleeping beauty mutagenesis screens
por: Riordan, Jesse D, et al.
Publicado: (2014)

Saturation Mutagenesis by Efficient Free-Energy Calculation
por: Jandova, Zuzana, et al.
Publicado: (2017)

Highly efficient libraries design for saturation mutagenesis
por: Pines, Gur, et al.
Publicado: (2022)

Hijacking Transposable Elements for Saturation Mutagenesis in Fungi
por: Schrevens, Sanne, et al.
Publicado: (2021)

A highly sensitive, PCR-based method for the detection of Plasmodium falciparum clones in microtiter plates
por: Maher, Steven P, et al.
Publicado: (2008)

FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method
por: Li, Chaokun, et al.
Publicado: (2011)

Site directed biotinylation of filamentous phage structural proteins
por: Smelyanski, Larisa, et al.
Publicado: (2011)

Deletion mutagenesis of large areas in Plasmodium falciparum genes: a comparative study
por: Williams, Marni, et al.
Publicado: (2007)

Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris
por: Tachioka, Mikako, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_9ft1kkqrvdrccktr0po23dr9rr