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Three-dimensional electron crystallography of protein microcrystals

We demonstrate that it is feasible to determine high-resolution protein structures by electron crystallography of three-dimensional crystals in an electron cryo-microscope (CryoEM). Lysozyme microcrystals were frozen on an electron microscopy grid, and electron diffraction data collected to 1.7 Å re...

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Detalles Bibliográficos
Autores principales: Shi, Dan, Nannenga, Brent L, Iadanza, Matthew G, Gonen, Tamir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3831942/
https://www.ncbi.nlm.nih.gov/pubmed/24252878
http://dx.doi.org/10.7554/eLife.01345