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Some factors affecting tannase production by Aspergillus niger Van Tieghem

One variable at a time procedure was used to evaluate the effect of qualitative variables on the production of tannase from Aspergillus niger Van Tieghem. These variables including: fermentation technique, agitation condition, tannins source, adding carbohydrates incorporation with tannic acid, nitr...

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Autores principales: Aboubakr, Hamada A., El-Sahn, Malak A., El-Banna, Amr A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Brazilian Society of Microbiology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833161/
https://www.ncbi.nlm.nih.gov/pubmed/24294255
http://dx.doi.org/10.1590/S1517-83822013000200036
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author Aboubakr, Hamada A.
El-Sahn, Malak A.
El-Banna, Amr A.
author_facet Aboubakr, Hamada A.
El-Sahn, Malak A.
El-Banna, Amr A.
author_sort Aboubakr, Hamada A.
collection PubMed
description One variable at a time procedure was used to evaluate the effect of qualitative variables on the production of tannase from Aspergillus niger Van Tieghem. These variables including: fermentation technique, agitation condition, tannins source, adding carbohydrates incorporation with tannic acid, nitrogen source type and divalent cations. Submerged fermentation under intermittent shaking gave the highest total tannase activity. Maximum extracellular tannase activity (305 units/50 mL) was attained in medium containing tannic acid as tannins source and sodium nitrate as nitrogen source at 30 °C for 96 h. All added carbohydrates showed significant adverse effects on the production of tannase. All tested divalent cations significantly decreased tannase production. Moreover, split plot design was carried out to study the effect of fermentation temperature and fermentation time on tannase production. The results indicated maximum tannase production (312.7 units/50 mL) at 35 °C for 96 h. In other words, increasing fermentation temperature from 30 °C to 35 °C resulted in increasing tannase production.
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spelling pubmed-38331612013-11-30 Some factors affecting tannase production by Aspergillus niger Van Tieghem Aboubakr, Hamada A. El-Sahn, Malak A. El-Banna, Amr A. Braz J Microbiol Research Paper One variable at a time procedure was used to evaluate the effect of qualitative variables on the production of tannase from Aspergillus niger Van Tieghem. These variables including: fermentation technique, agitation condition, tannins source, adding carbohydrates incorporation with tannic acid, nitrogen source type and divalent cations. Submerged fermentation under intermittent shaking gave the highest total tannase activity. Maximum extracellular tannase activity (305 units/50 mL) was attained in medium containing tannic acid as tannins source and sodium nitrate as nitrogen source at 30 °C for 96 h. All added carbohydrates showed significant adverse effects on the production of tannase. All tested divalent cations significantly decreased tannase production. Moreover, split plot design was carried out to study the effect of fermentation temperature and fermentation time on tannase production. The results indicated maximum tannase production (312.7 units/50 mL) at 35 °C for 96 h. In other words, increasing fermentation temperature from 30 °C to 35 °C resulted in increasing tannase production. Brazilian Society of Microbiology 2013-10-30 /pmc/articles/PMC3833161/ /pubmed/24294255 http://dx.doi.org/10.1590/S1517-83822013000200036 Text en Copyright © 2013, Sociedade Brasileira de Microbiologia All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Research Paper
Aboubakr, Hamada A.
El-Sahn, Malak A.
El-Banna, Amr A.
Some factors affecting tannase production by Aspergillus niger Van Tieghem
title Some factors affecting tannase production by Aspergillus niger Van Tieghem
title_full Some factors affecting tannase production by Aspergillus niger Van Tieghem
title_fullStr Some factors affecting tannase production by Aspergillus niger Van Tieghem
title_full_unstemmed Some factors affecting tannase production by Aspergillus niger Van Tieghem
title_short Some factors affecting tannase production by Aspergillus niger Van Tieghem
title_sort some factors affecting tannase production by aspergillus niger van tieghem
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3833161/
https://www.ncbi.nlm.nih.gov/pubmed/24294255
http://dx.doi.org/10.1590/S1517-83822013000200036
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