Increasing cleavage specificity and activity of restriction endonuclease KpnI

Restriction enzyme KpnI is a HNH superfamily endonuclease requiring divalent metal ions for DNA cleavage but not for binding. The active site of KpnI can accommodate metal ions of different atomic radii for DNA cleavage. Although Mg(2+) ion higher than 500 μM mediates promiscuous activity, Ca(2+) su...

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Detalles Bibliográficos
Autores principales: Vasu, Kommireddy, Nagamalleswari, Easa, Zahran, Mai, Imhof, Petra, Xu, Shuang-yong, Zhu, Zhenyu, Chan, Siu-Hong, Nagaraja, Valakunja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834813/
https://www.ncbi.nlm.nih.gov/pubmed/23963701
http://dx.doi.org/10.1093/nar/gkt734

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834813/
https://www.ncbi.nlm.nih.gov/pubmed/23963701
http://dx.doi.org/10.1093/nar/gkt734

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