The USP21 Short Variant (USP21SV) Lacking NES, Located Mostly in the Nucleus In Vivo, Activates Transcription by Deubiquitylating ubH2A In Vitro

USP21 is a deubiquitylase that catalyzes isopeptide bond hydrolysis between ubiquitin and histone H2A. Since ubiqutylated H2A (ubH2A) represses transcription, USP21 plays a role in transcriptional activation. On the other hand, the localization of USP21 suggests it has an additional function in the...

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Autores principales: Okuda, Hiroshi, Ohdan, Hideki, Nakayama, Manabu, Koseki, Haruhiko, Nakagawa, Takeya, Ito, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838379/
https://www.ncbi.nlm.nih.gov/pubmed/24278184
http://dx.doi.org/10.1371/journal.pone.0079813
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author Okuda, Hiroshi
Ohdan, Hideki
Nakayama, Manabu
Koseki, Haruhiko
Nakagawa, Takeya
Ito, Takashi
author_facet Okuda, Hiroshi
Ohdan, Hideki
Nakayama, Manabu
Koseki, Haruhiko
Nakagawa, Takeya
Ito, Takashi
author_sort Okuda, Hiroshi
collection PubMed
description USP21 is a deubiquitylase that catalyzes isopeptide bond hydrolysis between ubiquitin and histone H2A. Since ubiqutylated H2A (ubH2A) represses transcription, USP21 plays a role in transcriptional activation. On the other hand, the localization of USP21 suggests it has an additional function in the cytoplasm. Here, we identified a USP21 short variant (USP21SV) lacking a nuclear export signal (NES). Differential localization of USP21SV, more in the nucleus than the USP21 long variant (USP21LV), suggests they have redundant roles in the cell. Ectopic expression of both USP21 variants decreased ubH2A in the nucleus. Furthermore, both recombinant USP21 variants activate transcription by deubiquitylating ubH2A in vitro. These data suggest multiple roles for USP21 in the ubiquitylation-deubiquitylation network in the cell.
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spelling pubmed-38383792013-11-25 The USP21 Short Variant (USP21SV) Lacking NES, Located Mostly in the Nucleus In Vivo, Activates Transcription by Deubiquitylating ubH2A In Vitro Okuda, Hiroshi Ohdan, Hideki Nakayama, Manabu Koseki, Haruhiko Nakagawa, Takeya Ito, Takashi PLoS One Research Article USP21 is a deubiquitylase that catalyzes isopeptide bond hydrolysis between ubiquitin and histone H2A. Since ubiqutylated H2A (ubH2A) represses transcription, USP21 plays a role in transcriptional activation. On the other hand, the localization of USP21 suggests it has an additional function in the cytoplasm. Here, we identified a USP21 short variant (USP21SV) lacking a nuclear export signal (NES). Differential localization of USP21SV, more in the nucleus than the USP21 long variant (USP21LV), suggests they have redundant roles in the cell. Ectopic expression of both USP21 variants decreased ubH2A in the nucleus. Furthermore, both recombinant USP21 variants activate transcription by deubiquitylating ubH2A in vitro. These data suggest multiple roles for USP21 in the ubiquitylation-deubiquitylation network in the cell. Public Library of Science 2013-11-22 /pmc/articles/PMC3838379/ /pubmed/24278184 http://dx.doi.org/10.1371/journal.pone.0079813 Text en © 2013 Okuda et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Okuda, Hiroshi
Ohdan, Hideki
Nakayama, Manabu
Koseki, Haruhiko
Nakagawa, Takeya
Ito, Takashi
The USP21 Short Variant (USP21SV) Lacking NES, Located Mostly in the Nucleus In Vivo, Activates Transcription by Deubiquitylating ubH2A In Vitro
title The USP21 Short Variant (USP21SV) Lacking NES, Located Mostly in the Nucleus In Vivo, Activates Transcription by Deubiquitylating ubH2A In Vitro
title_full The USP21 Short Variant (USP21SV) Lacking NES, Located Mostly in the Nucleus In Vivo, Activates Transcription by Deubiquitylating ubH2A In Vitro
title_fullStr The USP21 Short Variant (USP21SV) Lacking NES, Located Mostly in the Nucleus In Vivo, Activates Transcription by Deubiquitylating ubH2A In Vitro
title_full_unstemmed The USP21 Short Variant (USP21SV) Lacking NES, Located Mostly in the Nucleus In Vivo, Activates Transcription by Deubiquitylating ubH2A In Vitro
title_short The USP21 Short Variant (USP21SV) Lacking NES, Located Mostly in the Nucleus In Vivo, Activates Transcription by Deubiquitylating ubH2A In Vitro
title_sort usp21 short variant (usp21sv) lacking nes, located mostly in the nucleus in vivo, activates transcription by deubiquitylating ubh2a in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838379/
https://www.ncbi.nlm.nih.gov/pubmed/24278184
http://dx.doi.org/10.1371/journal.pone.0079813
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