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PRC2 binds to active promoters and contacts nascent RNAs in embryonic stem cells

EZH2 is the catalytic subunit of PRC2, a central epigenetic repressor essential for development processes in vivo and for the differentiation of embryonic stem cells (ESCs) in vitro. The biochemical function of PRC2 in depositing repressive H3K27me3 marks is well understood, but how it is regulated...

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Detalles Bibliográficos
Autores principales: Kaneko, Syuzo, Son, Jinsook, Shen, Steven S., Reinberg, Danny, Bonasio, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839660/
https://www.ncbi.nlm.nih.gov/pubmed/24141703
http://dx.doi.org/10.1038/nsmb.2700
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author Kaneko, Syuzo
Son, Jinsook
Shen, Steven S.
Reinberg, Danny
Bonasio, Roberto
author_facet Kaneko, Syuzo
Son, Jinsook
Shen, Steven S.
Reinberg, Danny
Bonasio, Roberto
author_sort Kaneko, Syuzo
collection PubMed
description EZH2 is the catalytic subunit of PRC2, a central epigenetic repressor essential for development processes in vivo and for the differentiation of embryonic stem cells (ESCs) in vitro. The biochemical function of PRC2 in depositing repressive H3K27me3 marks is well understood, but how it is regulated and directed to specific genes before and during differentiation remains unknown. Here, we report that PRC2 binds at low levels to a majority of promoters in mouse ESCs, including many that are active and devoid of H3K27me3. Using in vivo RNA–protein crosslinking, we show that EZH2 directly binds to the 5′ of nascent RNAs transcribed from a subset of these promoters and that these binding events correlate with decreased H3K27me3. Our findings suggest a molecular mechanism by which PRC2 senses the transcriptional state of the cell and translates it into epigenetic information.
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spelling pubmed-38396602014-05-01 PRC2 binds to active promoters and contacts nascent RNAs in embryonic stem cells Kaneko, Syuzo Son, Jinsook Shen, Steven S. Reinberg, Danny Bonasio, Roberto Nat Struct Mol Biol Article EZH2 is the catalytic subunit of PRC2, a central epigenetic repressor essential for development processes in vivo and for the differentiation of embryonic stem cells (ESCs) in vitro. The biochemical function of PRC2 in depositing repressive H3K27me3 marks is well understood, but how it is regulated and directed to specific genes before and during differentiation remains unknown. Here, we report that PRC2 binds at low levels to a majority of promoters in mouse ESCs, including many that are active and devoid of H3K27me3. Using in vivo RNA–protein crosslinking, we show that EZH2 directly binds to the 5′ of nascent RNAs transcribed from a subset of these promoters and that these binding events correlate with decreased H3K27me3. Our findings suggest a molecular mechanism by which PRC2 senses the transcriptional state of the cell and translates it into epigenetic information. 2013-10-20 2013-11 /pmc/articles/PMC3839660/ /pubmed/24141703 http://dx.doi.org/10.1038/nsmb.2700 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Kaneko, Syuzo
Son, Jinsook
Shen, Steven S.
Reinberg, Danny
Bonasio, Roberto
PRC2 binds to active promoters and contacts nascent RNAs in embryonic stem cells
title PRC2 binds to active promoters and contacts nascent RNAs in embryonic stem cells
title_full PRC2 binds to active promoters and contacts nascent RNAs in embryonic stem cells
title_fullStr PRC2 binds to active promoters and contacts nascent RNAs in embryonic stem cells
title_full_unstemmed PRC2 binds to active promoters and contacts nascent RNAs in embryonic stem cells
title_short PRC2 binds to active promoters and contacts nascent RNAs in embryonic stem cells
title_sort prc2 binds to active promoters and contacts nascent rnas in embryonic stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839660/
https://www.ncbi.nlm.nih.gov/pubmed/24141703
http://dx.doi.org/10.1038/nsmb.2700
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