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Gene Expression Signature of Normal Cell-of-Origin Predicts Ovarian Tumor Outcomes
The potential role of the cell-of-origin in determining the tumor phenotype has been raised, but not adequately examined. We hypothesized that distinct cells-of-origin may play a role in determining ovarian tumor phenotype and outcome. Here we describe a new cell culture medium for in vitro culture...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3841174/ https://www.ncbi.nlm.nih.gov/pubmed/24303006 http://dx.doi.org/10.1371/journal.pone.0080314 |
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author | Merritt, Melissa A. Bentink, Stefan Schwede, Matthew Iwanicki, Marcin P. Quackenbush, John Woo, Terri Agoston, Elin S. Reinhardt, Ferenc Crum, Christopher P. Berkowitz, Ross S. Mok, Samuel C. Witt, Abigail E. Jones, Michelle A. Wang, Bin Ince, Tan A. |
author_facet | Merritt, Melissa A. Bentink, Stefan Schwede, Matthew Iwanicki, Marcin P. Quackenbush, John Woo, Terri Agoston, Elin S. Reinhardt, Ferenc Crum, Christopher P. Berkowitz, Ross S. Mok, Samuel C. Witt, Abigail E. Jones, Michelle A. Wang, Bin Ince, Tan A. |
author_sort | Merritt, Melissa A. |
collection | PubMed |
description | The potential role of the cell-of-origin in determining the tumor phenotype has been raised, but not adequately examined. We hypothesized that distinct cells-of-origin may play a role in determining ovarian tumor phenotype and outcome. Here we describe a new cell culture medium for in vitro culture of paired normal human ovarian (OV) and fallopian tube (FT) epithelial cells from donors without cancer. While these cells have been cultured individually for short periods of time, to our knowledge this is the first long-term culture of both cell types from the same donors. Through analysis of the gene expression profiles of the cultured OV/FT cells we identified a normal cell-of-origin gene signature that classified primary ovarian cancers into OV-like and FT-like subgroups; this classification correlated with significant differences in clinical outcomes. The identification of a prognostically significant gene expression signature derived solely from normal untransformed cells is consistent with the hypothesis that the normal cell-of-origin may be a source of ovarian tumor heterogeneity and the associated differences in tumor outcome. |
format | Online Article Text |
id | pubmed-3841174 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38411742013-12-03 Gene Expression Signature of Normal Cell-of-Origin Predicts Ovarian Tumor Outcomes Merritt, Melissa A. Bentink, Stefan Schwede, Matthew Iwanicki, Marcin P. Quackenbush, John Woo, Terri Agoston, Elin S. Reinhardt, Ferenc Crum, Christopher P. Berkowitz, Ross S. Mok, Samuel C. Witt, Abigail E. Jones, Michelle A. Wang, Bin Ince, Tan A. PLoS One Research Article The potential role of the cell-of-origin in determining the tumor phenotype has been raised, but not adequately examined. We hypothesized that distinct cells-of-origin may play a role in determining ovarian tumor phenotype and outcome. Here we describe a new cell culture medium for in vitro culture of paired normal human ovarian (OV) and fallopian tube (FT) epithelial cells from donors without cancer. While these cells have been cultured individually for short periods of time, to our knowledge this is the first long-term culture of both cell types from the same donors. Through analysis of the gene expression profiles of the cultured OV/FT cells we identified a normal cell-of-origin gene signature that classified primary ovarian cancers into OV-like and FT-like subgroups; this classification correlated with significant differences in clinical outcomes. The identification of a prognostically significant gene expression signature derived solely from normal untransformed cells is consistent with the hypothesis that the normal cell-of-origin may be a source of ovarian tumor heterogeneity and the associated differences in tumor outcome. Public Library of Science 2013-11-26 /pmc/articles/PMC3841174/ /pubmed/24303006 http://dx.doi.org/10.1371/journal.pone.0080314 Text en © 2013 Merritt et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Merritt, Melissa A. Bentink, Stefan Schwede, Matthew Iwanicki, Marcin P. Quackenbush, John Woo, Terri Agoston, Elin S. Reinhardt, Ferenc Crum, Christopher P. Berkowitz, Ross S. Mok, Samuel C. Witt, Abigail E. Jones, Michelle A. Wang, Bin Ince, Tan A. Gene Expression Signature of Normal Cell-of-Origin Predicts Ovarian Tumor Outcomes |
title | Gene Expression Signature of Normal Cell-of-Origin Predicts Ovarian Tumor Outcomes |
title_full | Gene Expression Signature of Normal Cell-of-Origin Predicts Ovarian Tumor Outcomes |
title_fullStr | Gene Expression Signature of Normal Cell-of-Origin Predicts Ovarian Tumor Outcomes |
title_full_unstemmed | Gene Expression Signature of Normal Cell-of-Origin Predicts Ovarian Tumor Outcomes |
title_short | Gene Expression Signature of Normal Cell-of-Origin Predicts Ovarian Tumor Outcomes |
title_sort | gene expression signature of normal cell-of-origin predicts ovarian tumor outcomes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3841174/ https://www.ncbi.nlm.nih.gov/pubmed/24303006 http://dx.doi.org/10.1371/journal.pone.0080314 |
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