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Biochemical and kinetic characterisation of a novel xylooligosaccharide-upregulated GH43 β-d-xylosidase/α-l-arabinofuranosidase (BXA43) from the probiotic Bifidobacterium animalis subsp. lactis BB-12

The Bifidobacterium animalis subsp. lactis BB-12 gene BIF_00092, assigned to encode a β-d-xylosidase (BXA43) of glycoside hydrolase family 43 (GH43), was cloned with a C-terminal His-tag and expressed in Escherichia coli. BXA43 was purified to homogeneity from the cell lysate and found to be a dual-...

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Detalles Bibliográficos
Autores principales: Viborg, Alexander Holm, Sørensen, Kim Ib, Gilad, Ofir, Steen-Jensen, Daniel Bisgaard, Dilokpimol, Adiphol, Jacobsen, Susanne, Svensson, Birte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3847938/
https://www.ncbi.nlm.nih.gov/pubmed/24025736
http://dx.doi.org/10.1186/2191-0855-3-56