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Characterization of Norovirus RNA replicase for in vitro amplification of RNA

BACKGROUND: The isothermal amplification of RNA in vitro has been used for the study of in vitro evolution of RNA. Although Qβ replicase has been traditionally used as an enzyme for this purpose, we planned to use norovirus replicase (NV3D(pol)) due to its structural simplicity in the scope of in vi...

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Detalles Bibliográficos
Autores principales: Arai, Hidenao, Nishigaki, Koichi, Nemoto, Naoto, Suzuki, Miho, Husimi, Yuzuru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852016/
https://www.ncbi.nlm.nih.gov/pubmed/24106810
http://dx.doi.org/10.1186/1472-6750-13-85