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Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration
The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotro...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852657/ https://www.ncbi.nlm.nih.gov/pubmed/24311593 http://dx.doi.org/10.1107/S0907444913025250 |
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author | Awad, Wael Svensson Birkedal, Gabriel Thunnissen, Marjolein M. G. M. Mani, Katrin Logan, Derek T. |
author_facet | Awad, Wael Svensson Birkedal, Gabriel Thunnissen, Marjolein M. G. M. Mani, Katrin Logan, Derek T. |
author_sort | Awad, Wael |
collection | PubMed |
description | The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotropy of crystals of the core protein of the human proteoglycan glypican-1. Crystals were subjected to controlled dehydration using the HC1 device. The optimal protocol for dehydration was developed by careful investigation of the following parameters: dehydration rate, final relative humidity and total incubation time T (inc). Of these, the most important was shown to be T (inc). After dehydration using the optimal protocol the crystals showed significantly reduced anisotropy and improved electron density, allowing the building of previously disordered parts of the structure. |
format | Online Article Text |
id | pubmed-3852657 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-38526572013-12-12 Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration Awad, Wael Svensson Birkedal, Gabriel Thunnissen, Marjolein M. G. M. Mani, Katrin Logan, Derek T. Acta Crystallogr D Biol Crystallogr Research Papers The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotropy of crystals of the core protein of the human proteoglycan glypican-1. Crystals were subjected to controlled dehydration using the HC1 device. The optimal protocol for dehydration was developed by careful investigation of the following parameters: dehydration rate, final relative humidity and total incubation time T (inc). Of these, the most important was shown to be T (inc). After dehydration using the optimal protocol the crystals showed significantly reduced anisotropy and improved electron density, allowing the building of previously disordered parts of the structure. International Union of Crystallography 2013-11-19 /pmc/articles/PMC3852657/ /pubmed/24311593 http://dx.doi.org/10.1107/S0907444913025250 Text en © Awad et al. 2013 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Awad, Wael Svensson Birkedal, Gabriel Thunnissen, Marjolein M. G. M. Mani, Katrin Logan, Derek T. Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration |
title | Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration |
title_full | Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration |
title_fullStr | Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration |
title_full_unstemmed | Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration |
title_short | Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration |
title_sort | improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852657/ https://www.ncbi.nlm.nih.gov/pubmed/24311593 http://dx.doi.org/10.1107/S0907444913025250 |
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