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Imaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio

The ATP:ADP ratio is a critical parameter of cellular energy status that regulates many metabolic activities. Here we report an optimized genetically-encoded fluorescent biosensor, PercevalHR, that senses the ATP:ADP ratio. PercevalHR is tuned to the range of intracellular ATP:ADP expected in mammal...

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Detalles Bibliográficos
Autores principales:	Tantama, Mathew, Martínez-François, Juan Ramón, Mongeon, Rebecca, Yellen, Gary
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2013
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3852917/ https://www.ncbi.nlm.nih.gov/pubmed/24096541 http://dx.doi.org/10.1038/ncomms3550

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