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Methanol fixation of plant tissue for Scanning Electron Microscopy improves preservation of tissue morphology and dimensions

BACKGROUND: It is well known that preparation of biological (plant and animal) tissues for Scanning Electron Microscopy (SEM) by chemical fixation and critical point drying results in shrinkage of tissues, often by up to 20-30%, depending on the tissue type and fixation protocol used. We sought to i...

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Detalles Bibliográficos
Autores principales: Talbot, Mark J, White, Rosemary G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3853006/
https://www.ncbi.nlm.nih.gov/pubmed/24083940
http://dx.doi.org/10.1186/1746-4811-9-36