Stability and magnetically induced heating behavior of lipid-coated Fe(3)O(4) nanoparticles

Magnetic nanoparticles that are currently explored for various biomedical applications exhibit a high propensity to minimize total surface energy through aggregation. This study introduces a unique, thermoresponsive nanocomposite design demonstrating substantial colloidal stability of superparamagne...

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Autores principales: Allam, Ayat A, Sadat, Md Ehsan, Potter, Sarah J, Mast, David B, Mohamed, Dina F, Habib, Fawzia S, Pauletti, Giovanni M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3853621/
https://www.ncbi.nlm.nih.gov/pubmed/24134544
http://dx.doi.org/10.1186/1556-276X-8-426
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author Allam, Ayat A
Sadat, Md Ehsan
Potter, Sarah J
Mast, David B
Mohamed, Dina F
Habib, Fawzia S
Pauletti, Giovanni M
author_facet Allam, Ayat A
Sadat, Md Ehsan
Potter, Sarah J
Mast, David B
Mohamed, Dina F
Habib, Fawzia S
Pauletti, Giovanni M
author_sort Allam, Ayat A
collection PubMed
description Magnetic nanoparticles that are currently explored for various biomedical applications exhibit a high propensity to minimize total surface energy through aggregation. This study introduces a unique, thermoresponsive nanocomposite design demonstrating substantial colloidal stability of superparamagnetic Fe(3)O(4) nanoparticles (SPIONs) due to a surface-immobilized lipid layer. Lipid coating was accomplished in different buffer systems, pH 7.4, using an equimolar mixture of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and l-α-dipalmitoylphosphatidyl glycerol (DPPG). Particle size and zeta potential were measured by dynamic laser light scattering. Heating behavior within an alternating magnetic field was compared between the commercial MFG-1000 magnetic field generator at 7 mT (1 MHz) and an experimental, laboratory-made magnetic hyperthermia system at 16.6 mT (13.7 MHz). The results revealed that product quality of lipid-coated SPIONs was significantly dependent on the colloidal stability of uncoated SPIONs during the coating process. Greatest stability was achieved at 0.02 mg/mL in citrate buffer (mean diameter = 80.0 ± 1.7 nm; zeta potential = -47.1 ± 2.6 mV). Surface immobilization of an equimolar DPPC/DPPG layer effectively reduced the impact of buffer components on particle aggregation. Most stable suspensions of lipid-coated nanoparticles were obtained at 0.02 mg/mL in citrate buffer (mean diameter = 179.3 ± 13.9 nm; zeta potential = -19.1 ± 2.3 mV). The configuration of the magnetic field generator significantly affected the heating properties of fabricated SPIONs. Heating rates of uncoated nanoparticles were substantially dependent on buffer composition but less influenced by particle concentration. In contrast, thermal behavior of lipid-coated nanoparticles within an alternating magnetic field was less influenced by suspension vehicle but dramatically more sensitive to particle concentration. These results underline the advantages of lipid-coated SPIONs on colloidal stability without compromising magnetically induced hyperthermia properties. Since phospholipids are biocompatible, these unique lipid-coated Fe(3)O(4) nanoparticles offer exciting opportunities as thermoresponsive drug delivery carriers for targeted, stimulus-induced therapeutic interventions. PACS: 7550Mw; 7575Cd; 8185Qr
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spelling pubmed-38536212013-12-11 Stability and magnetically induced heating behavior of lipid-coated Fe(3)O(4) nanoparticles Allam, Ayat A Sadat, Md Ehsan Potter, Sarah J Mast, David B Mohamed, Dina F Habib, Fawzia S Pauletti, Giovanni M Nanoscale Res Lett Nano Express Magnetic nanoparticles that are currently explored for various biomedical applications exhibit a high propensity to minimize total surface energy through aggregation. This study introduces a unique, thermoresponsive nanocomposite design demonstrating substantial colloidal stability of superparamagnetic Fe(3)O(4) nanoparticles (SPIONs) due to a surface-immobilized lipid layer. Lipid coating was accomplished in different buffer systems, pH 7.4, using an equimolar mixture of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and l-α-dipalmitoylphosphatidyl glycerol (DPPG). Particle size and zeta potential were measured by dynamic laser light scattering. Heating behavior within an alternating magnetic field was compared between the commercial MFG-1000 magnetic field generator at 7 mT (1 MHz) and an experimental, laboratory-made magnetic hyperthermia system at 16.6 mT (13.7 MHz). The results revealed that product quality of lipid-coated SPIONs was significantly dependent on the colloidal stability of uncoated SPIONs during the coating process. Greatest stability was achieved at 0.02 mg/mL in citrate buffer (mean diameter = 80.0 ± 1.7 nm; zeta potential = -47.1 ± 2.6 mV). Surface immobilization of an equimolar DPPC/DPPG layer effectively reduced the impact of buffer components on particle aggregation. Most stable suspensions of lipid-coated nanoparticles were obtained at 0.02 mg/mL in citrate buffer (mean diameter = 179.3 ± 13.9 nm; zeta potential = -19.1 ± 2.3 mV). The configuration of the magnetic field generator significantly affected the heating properties of fabricated SPIONs. Heating rates of uncoated nanoparticles were substantially dependent on buffer composition but less influenced by particle concentration. In contrast, thermal behavior of lipid-coated nanoparticles within an alternating magnetic field was less influenced by suspension vehicle but dramatically more sensitive to particle concentration. These results underline the advantages of lipid-coated SPIONs on colloidal stability without compromising magnetically induced hyperthermia properties. Since phospholipids are biocompatible, these unique lipid-coated Fe(3)O(4) nanoparticles offer exciting opportunities as thermoresponsive drug delivery carriers for targeted, stimulus-induced therapeutic interventions. PACS: 7550Mw; 7575Cd; 8185Qr Springer 2013-10-17 /pmc/articles/PMC3853621/ /pubmed/24134544 http://dx.doi.org/10.1186/1556-276X-8-426 Text en Copyright © 2013 Allam et al.; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Nano Express
Allam, Ayat A
Sadat, Md Ehsan
Potter, Sarah J
Mast, David B
Mohamed, Dina F
Habib, Fawzia S
Pauletti, Giovanni M
Stability and magnetically induced heating behavior of lipid-coated Fe(3)O(4) nanoparticles
title Stability and magnetically induced heating behavior of lipid-coated Fe(3)O(4) nanoparticles
title_full Stability and magnetically induced heating behavior of lipid-coated Fe(3)O(4) nanoparticles
title_fullStr Stability and magnetically induced heating behavior of lipid-coated Fe(3)O(4) nanoparticles
title_full_unstemmed Stability and magnetically induced heating behavior of lipid-coated Fe(3)O(4) nanoparticles
title_short Stability and magnetically induced heating behavior of lipid-coated Fe(3)O(4) nanoparticles
title_sort stability and magnetically induced heating behavior of lipid-coated fe(3)o(4) nanoparticles
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3853621/
https://www.ncbi.nlm.nih.gov/pubmed/24134544
http://dx.doi.org/10.1186/1556-276X-8-426
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