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Further characterisation of rotavirus cores: Ss(+)RNAs can be packaged in vitro but packaging lacks sequence specificity()

Rotavirus (RV) cores were released from double-layered particles (DLPs) by high concentrations of CaCl(2), purified and ‘opened’ by treatment with EDTA or EGTA. Under appropriate in vitro conditions DLPs have been shown to have transcriptase and ‘open cores’ replicase activity. Furthermore, it has b...

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Detalles Bibliográficos
Autores principales: Desselberger, Ulrich, Richards, James, Tchertanov, Luba, Lepault, Jean, Lever, Andrew, Burrone, Oscar, Cohen, Jean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854842/
https://www.ncbi.nlm.nih.gov/pubmed/24091366
http://dx.doi.org/10.1016/j.virusres.2013.09.034