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Plasma amino acids imbalance in cirrhotic patients disturbs the tricarboxylic acid cycle of dendritic cell
An imbalance of plasma amino acids (AA) is observed cirrhotic patients. Here we report that the imbalance suppresses the maturation of dendritic cells (DCs) by reducing the intracellular ATP due to interference with the mitochondrial tricarboxylic acid (TCA) cycle. We used serum-free culture medium...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857572/ https://www.ncbi.nlm.nih.gov/pubmed/24322372 http://dx.doi.org/10.1038/srep03459 |
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author | Kakazu, Eiji Kondo, Yasuteru Kogure, Takayuki Ninomiya, Masashi Kimura, Osamu Ueno, Yoshiyuki Shimosegawa, Tooru |
author_facet | Kakazu, Eiji Kondo, Yasuteru Kogure, Takayuki Ninomiya, Masashi Kimura, Osamu Ueno, Yoshiyuki Shimosegawa, Tooru |
author_sort | Kakazu, Eiji |
collection | PubMed |
description | An imbalance of plasma amino acids (AA) is observed cirrhotic patients. Here we report that the imbalance suppresses the maturation of dendritic cells (DCs) by reducing the intracellular ATP due to interference with the mitochondrial tricarboxylic acid (TCA) cycle. We used serum-free culture medium consistent with the average concentration of the plasma AA from a healthy volunteer (HCM) and that from patients with advanced cirrhosis (ACM). We compared the function of DCs and the metabolism of glucose-amino acids under each medium. The maturation and intracellular ATP of immature DCs were lower under ACM in spite of the enhancement of mitochondrial respiratory chain complex genes. Metabolomics revealed that the TCA cycle metabolite, fumarate and 2-oxoglutarate were increased in DCs generated under ACM. Consistent with in vitro, In CD1c(+) or CD14(+) cells from cirrhotic patients, the gene expression of 2-oxoglutarate-succinate-fumarate transition enzymes were significantly different from the cells of healthy controls. |
format | Online Article Text |
id | pubmed-3857572 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-38575722013-12-10 Plasma amino acids imbalance in cirrhotic patients disturbs the tricarboxylic acid cycle of dendritic cell Kakazu, Eiji Kondo, Yasuteru Kogure, Takayuki Ninomiya, Masashi Kimura, Osamu Ueno, Yoshiyuki Shimosegawa, Tooru Sci Rep Article An imbalance of plasma amino acids (AA) is observed cirrhotic patients. Here we report that the imbalance suppresses the maturation of dendritic cells (DCs) by reducing the intracellular ATP due to interference with the mitochondrial tricarboxylic acid (TCA) cycle. We used serum-free culture medium consistent with the average concentration of the plasma AA from a healthy volunteer (HCM) and that from patients with advanced cirrhosis (ACM). We compared the function of DCs and the metabolism of glucose-amino acids under each medium. The maturation and intracellular ATP of immature DCs were lower under ACM in spite of the enhancement of mitochondrial respiratory chain complex genes. Metabolomics revealed that the TCA cycle metabolite, fumarate and 2-oxoglutarate were increased in DCs generated under ACM. Consistent with in vitro, In CD1c(+) or CD14(+) cells from cirrhotic patients, the gene expression of 2-oxoglutarate-succinate-fumarate transition enzymes were significantly different from the cells of healthy controls. Nature Publishing Group 2013-12-10 /pmc/articles/PMC3857572/ /pubmed/24322372 http://dx.doi.org/10.1038/srep03459 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Article Kakazu, Eiji Kondo, Yasuteru Kogure, Takayuki Ninomiya, Masashi Kimura, Osamu Ueno, Yoshiyuki Shimosegawa, Tooru Plasma amino acids imbalance in cirrhotic patients disturbs the tricarboxylic acid cycle of dendritic cell |
title | Plasma amino acids imbalance in cirrhotic patients disturbs the tricarboxylic acid cycle of dendritic cell |
title_full | Plasma amino acids imbalance in cirrhotic patients disturbs the tricarboxylic acid cycle of dendritic cell |
title_fullStr | Plasma amino acids imbalance in cirrhotic patients disturbs the tricarboxylic acid cycle of dendritic cell |
title_full_unstemmed | Plasma amino acids imbalance in cirrhotic patients disturbs the tricarboxylic acid cycle of dendritic cell |
title_short | Plasma amino acids imbalance in cirrhotic patients disturbs the tricarboxylic acid cycle of dendritic cell |
title_sort | plasma amino acids imbalance in cirrhotic patients disturbs the tricarboxylic acid cycle of dendritic cell |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857572/ https://www.ncbi.nlm.nih.gov/pubmed/24322372 http://dx.doi.org/10.1038/srep03459 |
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