Simple Method for Shiga Toxin 2e Purification by Affinity Chromatography via Binding to the Divinyl Sulfone Group

Here we describe a simple affinity purification method for Shiga toxin 2e (Stx2e), a major causative factor of edema disease in swine. Escherichia coli strain MV1184 transformed with the expression plasmid pBSK-Stx2e produced Stx2e when cultivated in CAYE broth containing lincomycin. Stx2e bound to...

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Detalles Bibliográficos
Autores principales: Arimitsu, Hideyuki, Sasaki, Keiko, Kojima, Hiroe, Yanaka, Tadashi, Tsuji, Takao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858352/
https://www.ncbi.nlm.nih.gov/pubmed/24340102
http://dx.doi.org/10.1371/journal.pone.0083577

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858352/
https://www.ncbi.nlm.nih.gov/pubmed/24340102
http://dx.doi.org/10.1371/journal.pone.0083577

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