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Stable Agrobacterium-Mediated Transformation of Maritime Pine Based on Kanamycin Selection

An efficient transformation protocol based on kanamycin selection was developed for Agrobacterium-mediated transformation of maritime pine embryonal masses. The binary vector pBINUbiGUSint, which contained neomycin phosphotransferase II (nptII) as a selectable marker gene and β-glucuronidase (uidA)...

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Detalles Bibliográficos
Autores principales: Alvarez, José M., Ordás, Ricardo J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3859213/
https://www.ncbi.nlm.nih.gov/pubmed/24376383
http://dx.doi.org/10.1155/2013/681792