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Stable Agrobacterium-Mediated Transformation of Maritime Pine Based on Kanamycin Selection
An efficient transformation protocol based on kanamycin selection was developed for Agrobacterium-mediated transformation of maritime pine embryonal masses. The binary vector pBINUbiGUSint, which contained neomycin phosphotransferase II (nptII) as a selectable marker gene and β-glucuronidase (uidA)...
Autores principales: | Alvarez, José M., Ordás, Ricardo J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3859213/ https://www.ncbi.nlm.nih.gov/pubmed/24376383 http://dx.doi.org/10.1155/2013/681792 |
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