A comparison and optimization of methods and factors affecting the transformation of Escherichia coli

DNA manipulation routinely requires competent bacteria that can be made using one of numerous methods. To determine the best methods, we compared four commonly used chemical methods (DMSO, MgCl(2)–CaCl(2), CaCl(2) and Hanahan's methods) on frequently used Escherichia coli (E. coli) strains: DH5...

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Detalles Bibliográficos
Autores principales: Chan, Weng-Tat, Verma, Chandra S., Lane, David P., Gan, Samuel Ken-En
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2013
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3860579/
https://www.ncbi.nlm.nih.gov/pubmed/24229075
http://dx.doi.org/10.1042/BSR20130098

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3860579/
https://www.ncbi.nlm.nih.gov/pubmed/24229075
http://dx.doi.org/10.1042/BSR20130098

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