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A comparison and optimization of methods and factors affecting the transformation of Escherichia coli
DNA manipulation routinely requires competent bacteria that can be made using one of numerous methods. To determine the best methods, we compared four commonly used chemical methods (DMSO, MgCl(2)–CaCl(2), CaCl(2) and Hanahan's methods) on frequently used Escherichia coli (E. coli) strains: DH5...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Portland Press Ltd.
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3860579/ https://www.ncbi.nlm.nih.gov/pubmed/24229075 http://dx.doi.org/10.1042/BSR20130098 |