Cargando…

Immunization of Chlamydia pneumoniae (Cpn)-Infected Apob(tm2Sgy)Ldlr(tm1Her)/J Mice with a Combined Peptide of Cpn Significantly Reduces Atherosclerotic Lesion Development

OBJECTIVE: To investigate the antigenic effect of a peptide containing two epitopes of Chlamydia pneumoniae (Cpn) on atherosclerotic lesion formation in mice infected with Cpn. MATERIALS AND METHODS: Six-week-old Apob(tm2Sgy)Ldlr(tm1Her)/J mice were immunized using a repetitive immunization multiple...

Descripción completa

Detalles Bibliográficos
Autores principales: Xia, Min, Chen, Daxin, Endresz, Valeria, Faludi, Ildiko, Szabo, Andrea, Gonczol, Eva, Kakkar, Vijay, Lu, Xinjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3862476/
https://www.ncbi.nlm.nih.gov/pubmed/24349031
http://dx.doi.org/10.1371/journal.pone.0081056
_version_ 1782295742757470208
author Xia, Min
Chen, Daxin
Endresz, Valeria
Faludi, Ildiko
Szabo, Andrea
Gonczol, Eva
Kakkar, Vijay
Lu, Xinjie
author_facet Xia, Min
Chen, Daxin
Endresz, Valeria
Faludi, Ildiko
Szabo, Andrea
Gonczol, Eva
Kakkar, Vijay
Lu, Xinjie
author_sort Xia, Min
collection PubMed
description OBJECTIVE: To investigate the antigenic effect of a peptide containing two epitopes of Chlamydia pneumoniae (Cpn) on atherosclerotic lesion formation in mice infected with Cpn. MATERIALS AND METHODS: Six-week-old Apob(tm2Sgy)Ldlr(tm1Her)/J mice were immunized using a repetitive immunization multiple-sites strategy with KLH-conjugated peptides derived from the major outer membrane protein and the putative outer membrane protein 5 of Cpn. Mice were fed a high-fat diet and infected with Cpn twice during the 10-week diet period. Lesions were evaluated histologically; local and systemic immune responses were analyzed by immunohistochemistry of aorta samples and cytokine measurements in plasma samples and splenocyte supernatants. RESULTS: Mice immunized with the combined Cpn peptide showed a greater reduction in lesion size compared to mice immunized with either epitope alone [54.7% vs 39.8% or 41.72%] and was also associated with a significant decrease in lesion area in descending aortas compared with those in controls (88.9% for combined Cpn peptide, 81.9% for MOMP peptide and 75.7% for Omp5, respectively). This effect was associated with a shift in the cellular composition of plaques towards decreased inflammatory cell and increased regulatory T-cell content. Additionally, the effect was also connected with decreased secretion of proinflammatory cytokines and increased production of anti-inflammatory cytokines demonstrated in plasma and in supernatant on stimulated spleen cells. CONCLUSIONS: Atherosclerotic lesion formation may be promoted by Cpn infection in the presence of a high-fat diet, and reduced by immunization with the combined Cpn peptide. The combined peptide has more potential than either epitope alone in reducing atherosclerotic lesion development through Treg expansion.
format Online
Article
Text
id pubmed-3862476
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-38624762013-12-17 Immunization of Chlamydia pneumoniae (Cpn)-Infected Apob(tm2Sgy)Ldlr(tm1Her)/J Mice with a Combined Peptide of Cpn Significantly Reduces Atherosclerotic Lesion Development Xia, Min Chen, Daxin Endresz, Valeria Faludi, Ildiko Szabo, Andrea Gonczol, Eva Kakkar, Vijay Lu, Xinjie PLoS One Research Article OBJECTIVE: To investigate the antigenic effect of a peptide containing two epitopes of Chlamydia pneumoniae (Cpn) on atherosclerotic lesion formation in mice infected with Cpn. MATERIALS AND METHODS: Six-week-old Apob(tm2Sgy)Ldlr(tm1Her)/J mice were immunized using a repetitive immunization multiple-sites strategy with KLH-conjugated peptides derived from the major outer membrane protein and the putative outer membrane protein 5 of Cpn. Mice were fed a high-fat diet and infected with Cpn twice during the 10-week diet period. Lesions were evaluated histologically; local and systemic immune responses were analyzed by immunohistochemistry of aorta samples and cytokine measurements in plasma samples and splenocyte supernatants. RESULTS: Mice immunized with the combined Cpn peptide showed a greater reduction in lesion size compared to mice immunized with either epitope alone [54.7% vs 39.8% or 41.72%] and was also associated with a significant decrease in lesion area in descending aortas compared with those in controls (88.9% for combined Cpn peptide, 81.9% for MOMP peptide and 75.7% for Omp5, respectively). This effect was associated with a shift in the cellular composition of plaques towards decreased inflammatory cell and increased regulatory T-cell content. Additionally, the effect was also connected with decreased secretion of proinflammatory cytokines and increased production of anti-inflammatory cytokines demonstrated in plasma and in supernatant on stimulated spleen cells. CONCLUSIONS: Atherosclerotic lesion formation may be promoted by Cpn infection in the presence of a high-fat diet, and reduced by immunization with the combined Cpn peptide. The combined peptide has more potential than either epitope alone in reducing atherosclerotic lesion development through Treg expansion. Public Library of Science 2013-12-13 /pmc/articles/PMC3862476/ /pubmed/24349031 http://dx.doi.org/10.1371/journal.pone.0081056 Text en © 2013 Xia et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Xia, Min
Chen, Daxin
Endresz, Valeria
Faludi, Ildiko
Szabo, Andrea
Gonczol, Eva
Kakkar, Vijay
Lu, Xinjie
Immunization of Chlamydia pneumoniae (Cpn)-Infected Apob(tm2Sgy)Ldlr(tm1Her)/J Mice with a Combined Peptide of Cpn Significantly Reduces Atherosclerotic Lesion Development
title Immunization of Chlamydia pneumoniae (Cpn)-Infected Apob(tm2Sgy)Ldlr(tm1Her)/J Mice with a Combined Peptide of Cpn Significantly Reduces Atherosclerotic Lesion Development
title_full Immunization of Chlamydia pneumoniae (Cpn)-Infected Apob(tm2Sgy)Ldlr(tm1Her)/J Mice with a Combined Peptide of Cpn Significantly Reduces Atherosclerotic Lesion Development
title_fullStr Immunization of Chlamydia pneumoniae (Cpn)-Infected Apob(tm2Sgy)Ldlr(tm1Her)/J Mice with a Combined Peptide of Cpn Significantly Reduces Atherosclerotic Lesion Development
title_full_unstemmed Immunization of Chlamydia pneumoniae (Cpn)-Infected Apob(tm2Sgy)Ldlr(tm1Her)/J Mice with a Combined Peptide of Cpn Significantly Reduces Atherosclerotic Lesion Development
title_short Immunization of Chlamydia pneumoniae (Cpn)-Infected Apob(tm2Sgy)Ldlr(tm1Her)/J Mice with a Combined Peptide of Cpn Significantly Reduces Atherosclerotic Lesion Development
title_sort immunization of chlamydia pneumoniae (cpn)-infected apob(tm2sgy)ldlr(tm1her)/j mice with a combined peptide of cpn significantly reduces atherosclerotic lesion development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3862476/
https://www.ncbi.nlm.nih.gov/pubmed/24349031
http://dx.doi.org/10.1371/journal.pone.0081056
work_keys_str_mv AT xiamin immunizationofchlamydiapneumoniaecpninfectedapobtm2sgyldlrtm1herjmicewithacombinedpeptideofcpnsignificantlyreducesatheroscleroticlesiondevelopment
AT chendaxin immunizationofchlamydiapneumoniaecpninfectedapobtm2sgyldlrtm1herjmicewithacombinedpeptideofcpnsignificantlyreducesatheroscleroticlesiondevelopment
AT endreszvaleria immunizationofchlamydiapneumoniaecpninfectedapobtm2sgyldlrtm1herjmicewithacombinedpeptideofcpnsignificantlyreducesatheroscleroticlesiondevelopment
AT faludiildiko immunizationofchlamydiapneumoniaecpninfectedapobtm2sgyldlrtm1herjmicewithacombinedpeptideofcpnsignificantlyreducesatheroscleroticlesiondevelopment
AT szaboandrea immunizationofchlamydiapneumoniaecpninfectedapobtm2sgyldlrtm1herjmicewithacombinedpeptideofcpnsignificantlyreducesatheroscleroticlesiondevelopment
AT gonczoleva immunizationofchlamydiapneumoniaecpninfectedapobtm2sgyldlrtm1herjmicewithacombinedpeptideofcpnsignificantlyreducesatheroscleroticlesiondevelopment
AT kakkarvijay immunizationofchlamydiapneumoniaecpninfectedapobtm2sgyldlrtm1herjmicewithacombinedpeptideofcpnsignificantlyreducesatheroscleroticlesiondevelopment
AT luxinjie immunizationofchlamydiapneumoniaecpninfectedapobtm2sgyldlrtm1herjmicewithacombinedpeptideofcpnsignificantlyreducesatheroscleroticlesiondevelopment