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Hemodialysis does not alter in vitro hepatic CYP3A4 and CYP2D6 metabolic activity in uremic serum

There is a paucity of studies evaluating the change in liver metabolism in subjects receiving hemodialysis. The purpose of this study was to compare the effect of uremic toxins on hepatic cytochrome P450 (CYP)3A4 and CYP2D6 metabolism before and after a 4-hour hemodialysis session. Midazolam and dex...

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Autores principales: Decker, Brian S, O’Neill, Kalisha D, Chambers, Mary A, Slaven, James E, Yu, Zhangsheng, Jones, David R, Moe, Sharon M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3862653/
https://www.ncbi.nlm.nih.gov/pubmed/24353447
http://dx.doi.org/10.2147/CPAA.S54381
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author Decker, Brian S
O’Neill, Kalisha D
Chambers, Mary A
Slaven, James E
Yu, Zhangsheng
Jones, David R
Moe, Sharon M
author_facet Decker, Brian S
O’Neill, Kalisha D
Chambers, Mary A
Slaven, James E
Yu, Zhangsheng
Jones, David R
Moe, Sharon M
author_sort Decker, Brian S
collection PubMed
description There is a paucity of studies evaluating the change in liver metabolism in subjects receiving hemodialysis. The purpose of this study was to compare the effect of uremic toxins on hepatic cytochrome P450 (CYP)3A4 and CYP2D6 metabolism before and after a 4-hour hemodialysis session. Midazolam and dextromethorphan were incubated with uremic serum collected from subjects before and after the 4-hour hemodialysis session. Analysis and quantification of the 1′-OH-midazolam and 4-OH-midazolam and dextrorphan metabolites were performed by high-pressure liquid chromatography/mass spectrometry. Statistical analysis using the Student’s t-test (paired) was used to compare the amount of metabolite formed. The mean amount of 1′-OH-midazolam, 4-OH-midazolam, and dextrorphan metabolites formed before and after hemodialysis did not significantly differ. There was no significant difference in CYP3A4 and CYP2D6 metabolic activity in uremic serum before and after hemodialysis.
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spelling pubmed-38626532013-12-18 Hemodialysis does not alter in vitro hepatic CYP3A4 and CYP2D6 metabolic activity in uremic serum Decker, Brian S O’Neill, Kalisha D Chambers, Mary A Slaven, James E Yu, Zhangsheng Jones, David R Moe, Sharon M Clin Pharmacol Original Research There is a paucity of studies evaluating the change in liver metabolism in subjects receiving hemodialysis. The purpose of this study was to compare the effect of uremic toxins on hepatic cytochrome P450 (CYP)3A4 and CYP2D6 metabolism before and after a 4-hour hemodialysis session. Midazolam and dextromethorphan were incubated with uremic serum collected from subjects before and after the 4-hour hemodialysis session. Analysis and quantification of the 1′-OH-midazolam and 4-OH-midazolam and dextrorphan metabolites were performed by high-pressure liquid chromatography/mass spectrometry. Statistical analysis using the Student’s t-test (paired) was used to compare the amount of metabolite formed. The mean amount of 1′-OH-midazolam, 4-OH-midazolam, and dextrorphan metabolites formed before and after hemodialysis did not significantly differ. There was no significant difference in CYP3A4 and CYP2D6 metabolic activity in uremic serum before and after hemodialysis. Dove Medical Press 2013-12-10 /pmc/articles/PMC3862653/ /pubmed/24353447 http://dx.doi.org/10.2147/CPAA.S54381 Text en © 2013 Decker et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Decker, Brian S
O’Neill, Kalisha D
Chambers, Mary A
Slaven, James E
Yu, Zhangsheng
Jones, David R
Moe, Sharon M
Hemodialysis does not alter in vitro hepatic CYP3A4 and CYP2D6 metabolic activity in uremic serum
title Hemodialysis does not alter in vitro hepatic CYP3A4 and CYP2D6 metabolic activity in uremic serum
title_full Hemodialysis does not alter in vitro hepatic CYP3A4 and CYP2D6 metabolic activity in uremic serum
title_fullStr Hemodialysis does not alter in vitro hepatic CYP3A4 and CYP2D6 metabolic activity in uremic serum
title_full_unstemmed Hemodialysis does not alter in vitro hepatic CYP3A4 and CYP2D6 metabolic activity in uremic serum
title_short Hemodialysis does not alter in vitro hepatic CYP3A4 and CYP2D6 metabolic activity in uremic serum
title_sort hemodialysis does not alter in vitro hepatic cyp3a4 and cyp2d6 metabolic activity in uremic serum
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3862653/
https://www.ncbi.nlm.nih.gov/pubmed/24353447
http://dx.doi.org/10.2147/CPAA.S54381
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