Do-it-yourself histidine-tagged bovine enterokinase: A handy member of the protein engineer's toolbox()

Enterokinase, a two-chain duodenal serine protease, activates trypsinogen by removing its N-terminal propeptide. Due to a clean cut after the non-primed site recognition sequence, the enterokinase light chain is frequently employed in biotechnology to separate N-terminal affinity tags from target pr...

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Detalles Bibliográficos
Autores principales: Skala, Wolfgang, Goettig, Peter, Brandstetter, Hans
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science Publishers 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3863954/
https://www.ncbi.nlm.nih.gov/pubmed/24184090
http://dx.doi.org/10.1016/j.jbiotec.2013.10.022

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3863954/
https://www.ncbi.nlm.nih.gov/pubmed/24184090
http://dx.doi.org/10.1016/j.jbiotec.2013.10.022

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