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Do-it-yourself histidine-tagged bovine enterokinase: A handy member of the protein engineer's toolbox()
Enterokinase, a two-chain duodenal serine protease, activates trypsinogen by removing its N-terminal propeptide. Due to a clean cut after the non-primed site recognition sequence, the enterokinase light chain is frequently employed in biotechnology to separate N-terminal affinity tags from target pr...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier Science Publishers
2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3863954/ https://www.ncbi.nlm.nih.gov/pubmed/24184090 http://dx.doi.org/10.1016/j.jbiotec.2013.10.022 |