Development and validation of an approach to produce large‐scale quantities of CpG‐methylated plasmid DNA

The prokaryotic CpG‐specific DNA methylase from Spiroplasma, SssI methylase, has been extensively used to methylate plasmid DNA in vitro to investigate the effects of methylation in vertebrate systems. Currently available methods to produce CpG‐methylated plasmid DNA have certain limitations and can...

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Detalles Bibliográficos
Autor principal: Fletcher, Bradley S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3864432/
https://www.ncbi.nlm.nih.gov/pubmed/21261822
http://dx.doi.org/10.1111/j.1751-7915.2007.00007.x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3864432/
https://www.ncbi.nlm.nih.gov/pubmed/21261822
http://dx.doi.org/10.1111/j.1751-7915.2007.00007.x

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