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Development and validation of an approach to produce large‐scale quantities of CpG‐methylated plasmid DNA
The prokaryotic CpG‐specific DNA methylase from Spiroplasma, SssI methylase, has been extensively used to methylate plasmid DNA in vitro to investigate the effects of methylation in vertebrate systems. Currently available methods to produce CpG‐methylated plasmid DNA have certain limitations and can...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Blackwell Publishing Ltd
2008
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3864432/ https://www.ncbi.nlm.nih.gov/pubmed/21261822 http://dx.doi.org/10.1111/j.1751-7915.2007.00007.x |