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Mutant HbpR transcription activator isolation for 2‐chlorobiphenyl via green fluorescent protein‐based flow cytometry and cell sorting

Mutants were produced in the A‐domain of HbpR, a protein belonging to the XylR family of σ(54)‐dependent transcription activators, with the purpose of changing its effector recognition specificity from 2‐hydroxybiphenyl (2‐HBP, the cognate effector) to 2‐chlorobiphenyl (2‐CBP). Mutations were introd...

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Detalles Bibliográficos
Autores principales: Beggah, Siham, Vogne, Christelle, Zenaro, Elena, Van Der Meer, Jan Roelof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3864433/
https://www.ncbi.nlm.nih.gov/pubmed/21261823
http://dx.doi.org/10.1111/j.1751-7915.2007.00008.x