New vehicles allow detergent-free mass spectrometry of membrane protein complexes

The rapid growth in the study of membrane protein complexes in lipid microenvironments prompts new methods for their characterization. Here we develop mass spectrometry as a method for studying membrane proteins incorporated into amphipols, bicelles and nanodiscs. We compare these detergent-free veh...

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Autores principales: Hopper, Jonathan T.S., Yu, Yvonne Ting-Chun, Li, Dianfan, Raymond, Alison, Bostock, Mark, Liko, Idlir, Mikhailov, Victor, Laganowsky, Arthur, Benesch, Justin L.P., Caffrey, Martin, Nietlispach, Daniel, Robinson, Carol V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868940/
https://www.ncbi.nlm.nih.gov/pubmed/24122040
http://dx.doi.org/10.1038/nmeth.2691
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author Hopper, Jonathan T.S.
Yu, Yvonne Ting-Chun
Li, Dianfan
Raymond, Alison
Bostock, Mark
Liko, Idlir
Mikhailov, Victor
Laganowsky, Arthur
Benesch, Justin L.P.
Caffrey, Martin
Nietlispach, Daniel
Robinson, Carol V.
author_facet Hopper, Jonathan T.S.
Yu, Yvonne Ting-Chun
Li, Dianfan
Raymond, Alison
Bostock, Mark
Liko, Idlir
Mikhailov, Victor
Laganowsky, Arthur
Benesch, Justin L.P.
Caffrey, Martin
Nietlispach, Daniel
Robinson, Carol V.
author_sort Hopper, Jonathan T.S.
collection PubMed
description The rapid growth in the study of membrane protein complexes in lipid microenvironments prompts new methods for their characterization. Here we develop mass spectrometry as a method for studying membrane proteins incorporated into amphipols, bicelles and nanodiscs. We compare these detergent-free vehicles with micelles for their ability to preserve the interactions of oligomeric complexes and to stabilize proteins that require defined lipid environments.
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spelling pubmed-38689402014-06-01 New vehicles allow detergent-free mass spectrometry of membrane protein complexes Hopper, Jonathan T.S. Yu, Yvonne Ting-Chun Li, Dianfan Raymond, Alison Bostock, Mark Liko, Idlir Mikhailov, Victor Laganowsky, Arthur Benesch, Justin L.P. Caffrey, Martin Nietlispach, Daniel Robinson, Carol V. Nat Methods Article The rapid growth in the study of membrane protein complexes in lipid microenvironments prompts new methods for their characterization. Here we develop mass spectrometry as a method for studying membrane proteins incorporated into amphipols, bicelles and nanodiscs. We compare these detergent-free vehicles with micelles for their ability to preserve the interactions of oligomeric complexes and to stabilize proteins that require defined lipid environments. 2013-10-13 2013-12 /pmc/articles/PMC3868940/ /pubmed/24122040 http://dx.doi.org/10.1038/nmeth.2691 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Hopper, Jonathan T.S.
Yu, Yvonne Ting-Chun
Li, Dianfan
Raymond, Alison
Bostock, Mark
Liko, Idlir
Mikhailov, Victor
Laganowsky, Arthur
Benesch, Justin L.P.
Caffrey, Martin
Nietlispach, Daniel
Robinson, Carol V.
New vehicles allow detergent-free mass spectrometry of membrane protein complexes
title New vehicles allow detergent-free mass spectrometry of membrane protein complexes
title_full New vehicles allow detergent-free mass spectrometry of membrane protein complexes
title_fullStr New vehicles allow detergent-free mass spectrometry of membrane protein complexes
title_full_unstemmed New vehicles allow detergent-free mass spectrometry of membrane protein complexes
title_short New vehicles allow detergent-free mass spectrometry of membrane protein complexes
title_sort new vehicles allow detergent-free mass spectrometry of membrane protein complexes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3868940/
https://www.ncbi.nlm.nih.gov/pubmed/24122040
http://dx.doi.org/10.1038/nmeth.2691
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