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GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis
Building expression constructs for transgenesis is one of the fundamental day-to-day tasks in modern biology. Traditionally it is based on a multitude of type II restriction endonucleases and T4 DNA ligase. Especially in case of long inserts and applications requiring high-throughput, this approach...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869738/ https://www.ncbi.nlm.nih.gov/pubmed/24376629 http://dx.doi.org/10.1371/journal.pone.0083043 |