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Sequence-Defined Transposon Mutant Library of Burkholderia thailandensis

We constructed a near-saturation transposon mutant library for Burkholderia thailandensis, a low-virulence surrogate for the causative agent of melioidosis (Burkholderia pseudomallei). A primary set of nearly 42,000 unique mutants (~7.5 mutants/gene) was generated using transposon Tn5 derivatives. T...

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Detalles Bibliográficos
Autores principales: Gallagher, Larry A., Ramage, Elizabeth, Patrapuvich, Rapatbhorn, Weiss, Eli, Brittnacher, Mitch, Manoil, Colin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Microbiology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3870259/
https://www.ncbi.nlm.nih.gov/pubmed/24194535
http://dx.doi.org/10.1128/mBio.00604-13