A Practical Comparison of Ligation-Independent Cloning Techniques

The precise assembly of specific DNA sequences is a critical technique in molecular biology. Traditional cloning techniques use restriction enzymes and ligation of DNA in vitro, which can be hampered by a lack of appropriate restriction-sites and inefficient enzymatic steps. A number of ligation-ind...

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Detalles Bibliográficos
Autores principales: Stevenson, Julian, Krycer, James R., Phan, Lisa, Brown, Andrew J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871625/
https://www.ncbi.nlm.nih.gov/pubmed/24376768
http://dx.doi.org/10.1371/journal.pone.0083888

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871625/
https://www.ncbi.nlm.nih.gov/pubmed/24376768
http://dx.doi.org/10.1371/journal.pone.0083888

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