pH-based Detection of Phenylalnine by Potentiometric and Colorimetric Methods

In this paper, methods of sample preparation for potentiometric measurement of phenylalanine are presented. Basing on the spectrophotometric measurements of phenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe, 0,4 mM NAD(+), 2U L-PheDH) were determined. Then, the abs...

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Autores principales: Pijanowska, Dorota G., Remiszewska, El żbieta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2006
Materias:
Full Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3872363/
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author Pijanowska, Dorota G.
Remiszewska, El żbieta
author_facet Pijanowska, Dorota G.
Remiszewska, El żbieta
author_sort Pijanowska, Dorota G.
collection PubMed
description In this paper, methods of sample preparation for potentiometric measurement of phenylalanine are presented. Basing on the spectrophotometric measurements of phenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe, 0,4 mM NAD(+), 2U L-PheDH) were determined. Then, the absorption spectrum of the reaction product, NADH, was monitored (maximum peak at 340 nm). The results obtained by the spectrophotometric method were compared with the results obtained by the colourimetry, using pH indicators. The above-mentioned two methods will be used as references for potentiometric measurements of phenylalanine concentration.
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spelling pubmed-38723632013-12-26 pH-based Detection of Phenylalnine by Potentiometric and Colorimetric Methods Pijanowska, Dorota G. Remiszewska, El żbieta Sensors (Basel) Full Research Paper In this paper, methods of sample preparation for potentiometric measurement of phenylalanine are presented. Basing on the spectrophotometric measurements of phenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe, 0,4 mM NAD(+), 2U L-PheDH) were determined. Then, the absorption spectrum of the reaction product, NADH, was monitored (maximum peak at 340 nm). The results obtained by the spectrophotometric method were compared with the results obtained by the colourimetry, using pH indicators. The above-mentioned two methods will be used as references for potentiometric measurements of phenylalanine concentration. Molecular Diversity Preservation International (MDPI) 2006-04-07 /pmc/articles/PMC3872363/ Text en © 2006 by MDPI (http://www.mdpi.org). Reproduction is permitted for non-commercial purposes.
spellingShingle Full Research Paper
Pijanowska, Dorota G.
Remiszewska, El żbieta
pH-based Detection of Phenylalnine by Potentiometric and Colorimetric Methods
title pH-based Detection of Phenylalnine by Potentiometric and Colorimetric Methods
title_full pH-based Detection of Phenylalnine by Potentiometric and Colorimetric Methods
title_fullStr pH-based Detection of Phenylalnine by Potentiometric and Colorimetric Methods
title_full_unstemmed pH-based Detection of Phenylalnine by Potentiometric and Colorimetric Methods
title_short pH-based Detection of Phenylalnine by Potentiometric and Colorimetric Methods
title_sort ph-based detection of phenylalnine by potentiometric and colorimetric methods
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3872363/
work_keys_str_mv AT pijanowskadorotag phbaseddetectionofphenylalninebypotentiometricandcolorimetricmethods
AT remiszewskaelzbieta phbaseddetectionofphenylalninebypotentiometricandcolorimetricmethods

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