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Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly
In vitro recombination methods have enabled one-step construction of large DNA sequences from multiple parts. Although synthetic biological circuits can in principle be assembled in the same fashion, they typically contain repeated sequence elements such as standard promoters and terminators that in...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874176/ https://www.ncbi.nlm.nih.gov/pubmed/24078086 http://dx.doi.org/10.1093/nar/gkt860 |