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Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly

In vitro recombination methods have enabled one-step construction of large DNA sequences from multiple parts. Although synthetic biological circuits can in principle be assembled in the same fashion, they typically contain repeated sequence elements such as standard promoters and terminators that in...

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Detalles Bibliográficos
Autores principales:	Torella, Joseph P., Boehm, Christian R., Lienert, Florian, Chen, Jan-Hung, Way, Jeffrey C., Silver, Pamela A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Synthetic Biology and Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874176/ https://www.ncbi.nlm.nih.gov/pubmed/24078086 http://dx.doi.org/10.1093/nar/gkt860

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