One-pot DNA construction for synthetic biology: the Modular Overlap-Directed Assembly with Linkers (MODAL) strategy

Overlap-directed DNA assembly methods allow multiple DNA parts to be assembled together in one reaction. These methods, which rely on sequence homology between the ends of DNA parts, have become widely adopted in synthetic biology, despite being incompatible with a key principle of engineering: modu...

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Detalles Bibliográficos
Autores principales: Casini, Arturo, MacDonald, James T., Jonghe, Joachim De, Christodoulou, Georgia, Freemont, Paul S., Baldwin, Geoff S., Ellis, Tom
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874208/
https://www.ncbi.nlm.nih.gov/pubmed/24153110
http://dx.doi.org/10.1093/nar/gkt915

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874208/
https://www.ncbi.nlm.nih.gov/pubmed/24153110
http://dx.doi.org/10.1093/nar/gkt915

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