In Situ Overlap and Sequence Synthesis During DNA Assembly

[Image: see text] Modern cloning methods are independent from restriction enzyme recognition sites. However, nearly all current cloning methods still require the introduction of overlaps by PCR, which can introduce undesired mutations. Here, we investigated whether overlaps needed for DNA assembly c...

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Detalles Bibliográficos
Autores principales: Paetzold, Bernhard, Carolis, Carlo, Ferrar, Tony, Serrano, Luis, Lluch-Senar, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2013
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874219/
https://www.ncbi.nlm.nih.gov/pubmed/24161008
http://dx.doi.org/10.1021/sb400067v

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874219/
https://www.ncbi.nlm.nih.gov/pubmed/24161008
http://dx.doi.org/10.1021/sb400067v

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