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In Situ Overlap and Sequence Synthesis During DNA Assembly
[Image: see text] Modern cloning methods are independent from restriction enzyme recognition sites. However, nearly all current cloning methods still require the introduction of overlaps by PCR, which can introduce undesired mutations. Here, we investigated whether overlaps needed for DNA assembly c...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American
Chemical
Society
2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874219/ https://www.ncbi.nlm.nih.gov/pubmed/24161008 http://dx.doi.org/10.1021/sb400067v |