Consistent Errors in First Strand cDNA Due to Random Hexamer Mispriming

Priming of random hexamers in cDNA synthesis is known to show sequence bias, but in addition it has been suggested recently that mismatches in random hexamer priming could be a cause of mismatches between the original RNA fragment and observed sequence reads. To explore random hexamer mispriming as...

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Detalles Bibliográficos
Autores principales: van Gurp, Thomas P., McIntyre, Lauren M., Verhoeven, Koen J. F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875578/
https://www.ncbi.nlm.nih.gov/pubmed/24386481
http://dx.doi.org/10.1371/journal.pone.0085583

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875578/
https://www.ncbi.nlm.nih.gov/pubmed/24386481
http://dx.doi.org/10.1371/journal.pone.0085583

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