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Consistent Errors in First Strand cDNA Due to Random Hexamer Mispriming
Priming of random hexamers in cDNA synthesis is known to show sequence bias, but in addition it has been suggested recently that mismatches in random hexamer priming could be a cause of mismatches between the original RNA fragment and observed sequence reads. To explore random hexamer mispriming as...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875578/ https://www.ncbi.nlm.nih.gov/pubmed/24386481 http://dx.doi.org/10.1371/journal.pone.0085583 |