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Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair

Sequence-specific nucleases like TALENs and the CRISPR/Cas9 system have greatly expanded the genome editing possibilities in model organisms such as zebrafish. Both systems have recently been used to create knock-out alleles with great efficiency, and TALENs have also been successfully employed in k...

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Detalles Bibliográficos
Autores principales: Auer, Thomas O., Duroure, Karine, De Cian, Anne, Concordet, Jean-Paul, Del Bene, Filippo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3875856/
https://www.ncbi.nlm.nih.gov/pubmed/24179142
http://dx.doi.org/10.1101/gr.161638.113