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Light-Dependent and Circadian Transcription Dynamics In Vivo Recorded with a Destabilized Luciferase Reporter in Neurospora
We show that firefly luciferase is a stable protein when expressed at 25°C in Neurospora, which limits its use as transcription reporter. We created a short-lived luciferase by fusing a PEST signal to its C-terminus (LUC-PEST) and applied the LUC-PEST reporter system to record in vivo transcription...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3877077/ https://www.ncbi.nlm.nih.gov/pubmed/24391804 http://dx.doi.org/10.1371/journal.pone.0083660 |