Light-Dependent and Circadian Transcription Dynamics In Vivo Recorded with a Destabilized Luciferase Reporter in Neurospora

We show that firefly luciferase is a stable protein when expressed at 25°C in Neurospora, which limits its use as transcription reporter. We created a short-lived luciferase by fusing a PEST signal to its C-terminus (LUC-PEST) and applied the LUC-PEST reporter system to record in vivo transcription...

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Autores principales: Cesbron, François, Brunner, Michael, Diernfellner, Axel C. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3877077/
https://www.ncbi.nlm.nih.gov/pubmed/24391804
http://dx.doi.org/10.1371/journal.pone.0083660
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author Cesbron, François
Brunner, Michael
Diernfellner, Axel C. R.
author_facet Cesbron, François
Brunner, Michael
Diernfellner, Axel C. R.
author_sort Cesbron, François
collection PubMed
description We show that firefly luciferase is a stable protein when expressed at 25°C in Neurospora, which limits its use as transcription reporter. We created a short-lived luciferase by fusing a PEST signal to its C-terminus (LUC-PEST) and applied the LUC-PEST reporter system to record in vivo transcription dynamics associated with the Neurospora circadian clock and its blue-light photosensory system over the course of several days. We show that the tool is suitable to faithfully monitor rapid, but also subtle changes in transcription in a medium to high throughput format.
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spelling pubmed-38770772014-01-03 Light-Dependent and Circadian Transcription Dynamics In Vivo Recorded with a Destabilized Luciferase Reporter in Neurospora Cesbron, François Brunner, Michael Diernfellner, Axel C. R. PLoS One Research Article We show that firefly luciferase is a stable protein when expressed at 25°C in Neurospora, which limits its use as transcription reporter. We created a short-lived luciferase by fusing a PEST signal to its C-terminus (LUC-PEST) and applied the LUC-PEST reporter system to record in vivo transcription dynamics associated with the Neurospora circadian clock and its blue-light photosensory system over the course of several days. We show that the tool is suitable to faithfully monitor rapid, but also subtle changes in transcription in a medium to high throughput format. Public Library of Science 2013-12-31 /pmc/articles/PMC3877077/ /pubmed/24391804 http://dx.doi.org/10.1371/journal.pone.0083660 Text en © 2013 Cesbron et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cesbron, François
Brunner, Michael
Diernfellner, Axel C. R.
Light-Dependent and Circadian Transcription Dynamics In Vivo Recorded with a Destabilized Luciferase Reporter in Neurospora
title Light-Dependent and Circadian Transcription Dynamics In Vivo Recorded with a Destabilized Luciferase Reporter in Neurospora
title_full Light-Dependent and Circadian Transcription Dynamics In Vivo Recorded with a Destabilized Luciferase Reporter in Neurospora
title_fullStr Light-Dependent and Circadian Transcription Dynamics In Vivo Recorded with a Destabilized Luciferase Reporter in Neurospora
title_full_unstemmed Light-Dependent and Circadian Transcription Dynamics In Vivo Recorded with a Destabilized Luciferase Reporter in Neurospora
title_short Light-Dependent and Circadian Transcription Dynamics In Vivo Recorded with a Destabilized Luciferase Reporter in Neurospora
title_sort light-dependent and circadian transcription dynamics in vivo recorded with a destabilized luciferase reporter in neurospora
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3877077/
https://www.ncbi.nlm.nih.gov/pubmed/24391804
http://dx.doi.org/10.1371/journal.pone.0083660
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AT diernfellneraxelcr lightdependentandcircadiantranscriptiondynamicsinvivorecordedwithadestabilizedluciferasereporterinneurospora

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