Quantifying RNA allelic ratios by microfluidics-based multiplex PCR and deep sequencing

We developed a targeted RNA sequencing method that couples microfluidics-based multiplex PCR and deep sequencing (mmPCR-seq) to uniformly and simultaneously amplify up to 960 loci in 48 samples independently of their gene expression levels, and accurately and cost-effectively measure allelic ratios...

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Detalles Bibliográficos
Autores principales: Zhang, Rui, Li, Xin, Ramaswami, Gokul, Smith, Kevin S, Turecki, Gustavo, Montgomery, Stephen B, Li, Jin Billy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3877737/
https://www.ncbi.nlm.nih.gov/pubmed/24270603
http://dx.doi.org/10.1038/nmeth.2736

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3877737/
https://www.ncbi.nlm.nih.gov/pubmed/24270603
http://dx.doi.org/10.1038/nmeth.2736

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