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Quantifying RNA allelic ratios by microfluidics-based multiplex PCR and deep sequencing
We developed a targeted RNA sequencing method that couples microfluidics-based multiplex PCR and deep sequencing (mmPCR-seq) to uniformly and simultaneously amplify up to 960 loci in 48 samples independently of their gene expression levels, and accurately and cost-effectively measure allelic ratios...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3877737/ https://www.ncbi.nlm.nih.gov/pubmed/24270603 http://dx.doi.org/10.1038/nmeth.2736 |
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author | Zhang, Rui Li, Xin Ramaswami, Gokul Smith, Kevin S Turecki, Gustavo Montgomery, Stephen B Li, Jin Billy |
author_facet | Zhang, Rui Li, Xin Ramaswami, Gokul Smith, Kevin S Turecki, Gustavo Montgomery, Stephen B Li, Jin Billy |
author_sort | Zhang, Rui |
collection | PubMed |
description | We developed a targeted RNA sequencing method that couples microfluidics-based multiplex PCR and deep sequencing (mmPCR-seq) to uniformly and simultaneously amplify up to 960 loci in 48 samples independently of their gene expression levels, and accurately and cost-effectively measure allelic ratios even for low-quantity or low-quality RNA samples. We applied mmPCR-seq to RNA editing and allele-specific expression studies. mmPCR-seq complements RNA-seq and provides a highly desirable solution for future applications. |
format | Online Article Text |
id | pubmed-3877737 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
record_format | MEDLINE/PubMed |
spelling | pubmed-38777372014-07-01 Quantifying RNA allelic ratios by microfluidics-based multiplex PCR and deep sequencing Zhang, Rui Li, Xin Ramaswami, Gokul Smith, Kevin S Turecki, Gustavo Montgomery, Stephen B Li, Jin Billy Nat Methods Article We developed a targeted RNA sequencing method that couples microfluidics-based multiplex PCR and deep sequencing (mmPCR-seq) to uniformly and simultaneously amplify up to 960 loci in 48 samples independently of their gene expression levels, and accurately and cost-effectively measure allelic ratios even for low-quantity or low-quality RNA samples. We applied mmPCR-seq to RNA editing and allele-specific expression studies. mmPCR-seq complements RNA-seq and provides a highly desirable solution for future applications. 2013-11-24 2014-01 /pmc/articles/PMC3877737/ /pubmed/24270603 http://dx.doi.org/10.1038/nmeth.2736 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Zhang, Rui Li, Xin Ramaswami, Gokul Smith, Kevin S Turecki, Gustavo Montgomery, Stephen B Li, Jin Billy Quantifying RNA allelic ratios by microfluidics-based multiplex PCR and deep sequencing |
title | Quantifying RNA allelic ratios by microfluidics-based multiplex PCR and deep sequencing |
title_full | Quantifying RNA allelic ratios by microfluidics-based multiplex PCR and deep sequencing |
title_fullStr | Quantifying RNA allelic ratios by microfluidics-based multiplex PCR and deep sequencing |
title_full_unstemmed | Quantifying RNA allelic ratios by microfluidics-based multiplex PCR and deep sequencing |
title_short | Quantifying RNA allelic ratios by microfluidics-based multiplex PCR and deep sequencing |
title_sort | quantifying rna allelic ratios by microfluidics-based multiplex pcr and deep sequencing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3877737/ https://www.ncbi.nlm.nih.gov/pubmed/24270603 http://dx.doi.org/10.1038/nmeth.2736 |
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