Cargando…
Quantifying RNA allelic ratios by microfluidics-based multiplex PCR and deep sequencing
We developed a targeted RNA sequencing method that couples microfluidics-based multiplex PCR and deep sequencing (mmPCR-seq) to uniformly and simultaneously amplify up to 960 loci in 48 samples independently of their gene expression levels, and accurately and cost-effectively measure allelic ratios...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2013
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3877737/ https://www.ncbi.nlm.nih.gov/pubmed/24270603 http://dx.doi.org/10.1038/nmeth.2736 |