A universal TaqMan-based RT-PCR protocol for cost-efficient detection of small noncoding RNA

Several methods for the detection of RNA have been developed over time. For small RNA detection, a stem–loop reverse primer-based protocol relying on TaqMan RT-PCR has been described. This protocol requires an individual specific TaqMan probe for each target RNA and, hence, is highly cost-intensive...

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Detalles Bibliográficos
Autores principales: Jung, Ulrike, Jiang, Xiaoou, Kaufmann, Stefan H.E., Patzel, Volker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2013
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3884658/
https://www.ncbi.nlm.nih.gov/pubmed/24149841
http://dx.doi.org/10.1261/rna.040501.113

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3884658/
https://www.ncbi.nlm.nih.gov/pubmed/24149841
http://dx.doi.org/10.1261/rna.040501.113

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