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A universal TaqMan-based RT-PCR protocol for cost-efficient detection of small noncoding RNA
Several methods for the detection of RNA have been developed over time. For small RNA detection, a stem–loop reverse primer-based protocol relying on TaqMan RT-PCR has been described. This protocol requires an individual specific TaqMan probe for each target RNA and, hence, is highly cost-intensive...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Cold Spring Harbor Laboratory Press
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3884658/ https://www.ncbi.nlm.nih.gov/pubmed/24149841 http://dx.doi.org/10.1261/rna.040501.113 |