Cargando…

Resveratrol Induces Vascular Smooth Muscle Cell Differentiation through Stimulation of SirT1 and AMPK

Phenotypic plasticity in vascular smooth muscle cells (VSMC) is necessary for vessel maintenance, repair and adaptation to vascular changes associated with aging. De-differentiated VSMC contribute to pathologies including atherosclerosis and intimal hyperplasia. As resveratrol has been reported to h...

Descripción completa

Detalles Bibliográficos
Autores principales: Thompson, Anne Marie, Martin, Kathleen A., Rzucidlo, Eva M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885718/
https://www.ncbi.nlm.nih.gov/pubmed/24416418
http://dx.doi.org/10.1371/journal.pone.0085495
_version_ 1782298805831467008
author Thompson, Anne Marie
Martin, Kathleen A.
Rzucidlo, Eva M.
author_facet Thompson, Anne Marie
Martin, Kathleen A.
Rzucidlo, Eva M.
author_sort Thompson, Anne Marie
collection PubMed
description Phenotypic plasticity in vascular smooth muscle cells (VSMC) is necessary for vessel maintenance, repair and adaptation to vascular changes associated with aging. De-differentiated VSMC contribute to pathologies including atherosclerosis and intimal hyperplasia. As resveratrol has been reported to have cardio- protective effects, we investigated its role in VSMC phenotypic modulation. We demonstrated the novel finding that resveratrol promoted VSMC differentiation as measured by contractile protein expression, contractile morphology and contraction in collagen gels. Resveratrol induced VSMC differentiation through stimulation of SirT1 and AMPK. We made the novel finding that low or high dose resveratrol had an initially different mechanism on induction of differentiation. We found that low dose resveratrol stimulated differentiation through SirT1-mediated activation of AKT, whereas high dose resveratrol stimulated differentiation through AMPK-mediated inhibition of the mTORC1 pathway, allowing activation of AKT. The health effects of resveratrol in cardiovascular diseases, cancer and longevity are an area of active research. We have demonstrated a supplemental avenue where-by resveratrol may promote health by maintaining and enhancing plasticity of the vasculature.
format Online
Article
Text
id pubmed-3885718
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-38857182014-01-10 Resveratrol Induces Vascular Smooth Muscle Cell Differentiation through Stimulation of SirT1 and AMPK Thompson, Anne Marie Martin, Kathleen A. Rzucidlo, Eva M. PLoS One Research Article Phenotypic plasticity in vascular smooth muscle cells (VSMC) is necessary for vessel maintenance, repair and adaptation to vascular changes associated with aging. De-differentiated VSMC contribute to pathologies including atherosclerosis and intimal hyperplasia. As resveratrol has been reported to have cardio- protective effects, we investigated its role in VSMC phenotypic modulation. We demonstrated the novel finding that resveratrol promoted VSMC differentiation as measured by contractile protein expression, contractile morphology and contraction in collagen gels. Resveratrol induced VSMC differentiation through stimulation of SirT1 and AMPK. We made the novel finding that low or high dose resveratrol had an initially different mechanism on induction of differentiation. We found that low dose resveratrol stimulated differentiation through SirT1-mediated activation of AKT, whereas high dose resveratrol stimulated differentiation through AMPK-mediated inhibition of the mTORC1 pathway, allowing activation of AKT. The health effects of resveratrol in cardiovascular diseases, cancer and longevity are an area of active research. We have demonstrated a supplemental avenue where-by resveratrol may promote health by maintaining and enhancing plasticity of the vasculature. Public Library of Science 2014-01-08 /pmc/articles/PMC3885718/ /pubmed/24416418 http://dx.doi.org/10.1371/journal.pone.0085495 Text en © 2014 Thompson et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Thompson, Anne Marie
Martin, Kathleen A.
Rzucidlo, Eva M.
Resveratrol Induces Vascular Smooth Muscle Cell Differentiation through Stimulation of SirT1 and AMPK
title Resveratrol Induces Vascular Smooth Muscle Cell Differentiation through Stimulation of SirT1 and AMPK
title_full Resveratrol Induces Vascular Smooth Muscle Cell Differentiation through Stimulation of SirT1 and AMPK
title_fullStr Resveratrol Induces Vascular Smooth Muscle Cell Differentiation through Stimulation of SirT1 and AMPK
title_full_unstemmed Resveratrol Induces Vascular Smooth Muscle Cell Differentiation through Stimulation of SirT1 and AMPK
title_short Resveratrol Induces Vascular Smooth Muscle Cell Differentiation through Stimulation of SirT1 and AMPK
title_sort resveratrol induces vascular smooth muscle cell differentiation through stimulation of sirt1 and ampk
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885718/
https://www.ncbi.nlm.nih.gov/pubmed/24416418
http://dx.doi.org/10.1371/journal.pone.0085495
work_keys_str_mv AT thompsonannemarie resveratrolinducesvascularsmoothmusclecelldifferentiationthroughstimulationofsirt1andampk
AT martinkathleena resveratrolinducesvascularsmoothmusclecelldifferentiationthroughstimulationofsirt1andampk
AT rzucidloevam resveratrolinducesvascularsmoothmusclecelldifferentiationthroughstimulationofsirt1andampk